A Level Biology - Gene Technology Crossword Puzzle

QUESTIONS LIST:

• restriction enzyme : enzyme that cuts dna at specific recognition sequences
• plasmid : small circular dna molecule used as a vector in genetic engineering
• ligase : enzyme that joins dna fragments together
• vector : dna molecule used to transfer a gene into a host cell
• recombinant dna : dna formed by combining genetic material from different organisms
• host cell : cell that receives recombinant dna
• antibiotic resistance : marker gene used to identify transformed bacteria
• promoter : dna sequence that initiates transcription of a gene
• reverse transcriptase : enzyme that synthesises dna from an rna template
• cdna : dna produced from mrna using reverse transcriptase
• pcr : technique used to amplify dna in vitro
• taq polymerase : heat-stable enzyme used in pcr
• denaturation : step in pcr where double-stranded dna separates
• annealing : step in pcr where primers bind to dna templates
• gel electrophoresis : method using an electric field to separate charged molecules
• probe : labelled dna fragment used to identify specific sequences