Biotechnology Crossword Puzzle

QUESTIONS LIST:

• pcr : denaturation; annealing, extending (abbr.)
• crispr : recently developed method for making precise and targeted changes in the genome of a living cell
• forward genetics : beginning with a phenotype, and ending with a gene that encodes that phenotype.
• western : if its not southern, or northern, but it does have protein it's...
• gene therapy : direct transfer of genes into humans to treat disease.
• taqpolymerase : doesn't break down in extremely high temperatures, so you know it'll get attached...
• gel electrophoresis : some gel, in wells, and dna fragments that are small as he...
• southern : blotting used to detect deoxyribonucleic acid
• northern : blotting used to detect not deoxyribonucleic acid, but...
• dna library : probably filled with so many "books" i'll never read; you might, if you're into that kind of stuff...
• biotechnology : using techniques to develop new products
• cloning vector : an origin of replication; selectable markers; one or more unique restriction sites.
• dna fingerprinting : better hope you don't end up in codis...
• reverse genetics : using a gene of unknown function to induce a mutation, then study the effect of the mutation on the phenotype of an organism
• micro satellites : very short dna sequences repeated in tandem.
• restriction enzyme : ecori, hindiii, bamhi, pvuii...
• probe : dna or rna with a base sequence complementary to a sequence in the gene of interest